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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 14, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Detection of multiple SNPs in numerous samples with polyacrylamide gel-based microarray.

Lu Cheng¹, Beili Sun, Yu Sun

¹State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.

Journal of Nanoscience and Nanotechnology

|April 1, 2010

Summary

This summary is machine-generated.

This study enhances gel-based DNA microarrays for high-throughput genotyping of multiple single nucleotide polymorphisms (SNPs). The improved method, using multiplex-PCR and dual-color detectors, is efficient, cost-effective, and time-saving for SNP analysis.

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Last Updated: Jun 14, 2026

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Developed a robust polyacrylamide gel-based DNA microarray for high-throughput single nucleotide polymorphism (SNP) screening.
Previous methods were effective for large-scale SNP genotyping but limited to single SNP analysis per sample.

Purpose of the Study:

To improve the existing gel-based DNA microarray platform for simultaneous detection of multiple SNPs.
To enhance efficiency and reduce costs associated with high-throughput SNP genotyping.

Main Methods:

Integrated multiplex polymerase chain reaction (multiplex-PCR) to amplify multiple SNP targets simultaneously.
Immobilized multiplex-PCR products onto a 3-dimensional polyacrylamide gel matrix.
Utilized universal dual-color fluorescent detectors for hybridization and detection.

Main Results:

Successfully genotyped five specific SNPs (rs191296, rs2280073, rs17599165, rs17599416, rs7660336) in the GABRA4 gene using the improved platform.
Demonstrated significant labor and time savings compared to single SNP analysis.
Achieved over a two-thirds cost reduction for multiple SNP analysis by employing universal dual-color fluorescent detectors.

Conclusions:

The combination of multiplex-PCR and gel-based microarray with universal dual-color fluorescent detectors offers an efficient, rapid, and simple method for detecting single nucleotide mismatches.
This optimized platform presents a competitive approach for DNA microarray construction in terms of efficiency, fidelity, and cost-effectiveness.