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Updated: Jun 14, 2026
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Electrochemical biosensor microarray functionalized by means of biomolecule friendly photolithography
Mònica Mir1, Srujan Kumar Dondapati, Maria Viviana Duarte
1Bioengineering and Bioelectrochemistry Group, Departament d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona 43007, Spain. mmir@ibec.pcb.ub.es
This study introduces a photolithographic method for selectively functionalizing microelectrodes, enabling multi-analyte biosensor arrays. This technique allows for the precise immobilization of diverse biomolecules for advanced diagnostics.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Microfabrication enables dense electrode arrays for diagnostics.
- Selective functionalization of microelectrodes with biomolecules is challenging.
- Developing multi-analyte biosensor arrays requires precise control over biomolecule immobilization.
Purpose of the Study:
- To address the challenge of selectively functionalizing arbitrary shape microelectrodes.
- To develop a photolithographic procedure for sequential immobilization of different biomolecules onto separated electrodes.
- To demonstrate the fabrication of multi-analyte biosensor arrays using this method.
Main Methods:
- Employed a biomolecule-friendly photolithographic procedure.
- Sequentially immobilized different biomolecules onto separated electrodes of the same array.
- Utilized electrochemical techniques for detection in a two-analyte array format.
Main Results:
- Successfully demonstrated selective detection of oligonucleotides, hormone T4, sarcosine, and glucose.
- Validated compatibility with various biorecognition molecules including DNA, antibodies, and enzymes.
- Achieved selective modification of closely spaced microelectrodes (20 microm sensor diameter and 20 microm-spaced interdigitated electrodes array).
Conclusions:
- The proposed photolithographic method is effective for fabricating multi-analyte biosensor arrays.
- The method is generic and compatible with diverse biomolecules.
- This approach holds potential for patterning electrochemical multi-analyte biosensors at higher resolutions.
