Related Experiment Video
Updated: Jun 14, 2026
Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
Published on: May 9, 2025
Is cytology reliable for epidermal growth factor receptor gene evaluation in non-small cell lung cancer?
Cecilia Bozzetti1, Marcello Tiseo, Costanza Lagrasta
1Oncologia Medica, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy.
Background:
Epidermal growth factor receptor (EGFR) gene copy number has been proposed as predictor of response to epidermal growth factor receptor tyrosine kinase inhibitors in advanced non-small cell lung cancer (NSCLC).
Methods:
Cytologic and matched histologic samples from 33 primary non-small cell lung cancers were analyzed by fluorescence in situ hybridization (FISH) for epidermal growth factor receptor gene.
Results:
FISH was positive in 52% and negative in 35% of the 31 matched evaluable samples. Four of 31 (13%) cases were discordant (K = 0.736; p < 0.001).
Conclusion:
Our data support the feasibility and reliability of epidermal growth factor receptor gene assessment by FISH on cytology.
