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Updated: Jun 14, 2026

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
[Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation
Zhi-qiang Chen1, Yun-bei Li, Qin-xue Wen
1National Engineering Research Center of Urban Water Resources, Harbin 150090, China. czq0521@tom.com
Abstract:
A bacterial strain,WD-3,with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH,fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g x L(-), the strain accumulated PHA up to 45.4% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.
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