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Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components
A Elsner1, F Löffler, K Miyashita
1Institut für Mikrobiologie, Universität Hohenheim, Stuttgart, Federal Republic of Germany.
Applied and Environmental Microbiology
|January 1, 1991
Abstract:
Extracts of Pseudomonas sp. strain CBS3 grown with 4-chlorobenzoate as sole carbon source contained an enzyme that converted 4-chlorobenzoate to 4-hydroxybenzoate. This enzyme was shown to consist of three components, all necessary for the reaction. Component I, which had a molecular weight of about 3,000, was highly unstable. Components II and III were stable proteins with molecular weights of about 86,000 and 92,000.