[Biopanning of HCV antigen epitopes from a random 12-peptide library by anti-hepatitis C virus polyclonal antibody]
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Updated: Jun 14, 2026
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
Min Wang1, Xianping Li, Qinglin Wang
1Department of Clinical Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China. wmli2007@yahoo.cn
Objective:
To analyze the epitopes of anti-hepatitis C virus(HCV)antibodies by peptide library biopanning.
Methods:
Phage random peptide library of 12 amino acids was immunoscreened with purified IgG from the sera of hepatitis C patients. Positive clones which were obtained after 3 rounds of biopanning were detected by ELISA and 4 of them were sequenced.
Results:
After 3 rounds of screening, the radio of output to input increased from (4.6 x 10(-4))% to (5.3 x 10(-2))%, meaning the enrichment was effective. At the third round of screening, all the selected clones proved to specifically react with the sera for immunoscreening. Four positive phage clones were sequenced, which shared a very conservative sequence and was named as C1. Its inserting sequence in the coat protein III was deduced to be GSMSPYVRWYTP, and the positive rate of C1 reacted with 20 cases of HCV patients was 85%.
Conclusion:
The antigen-mimic peptide C1 is successfully screened from 12 random phage peptide library and it has some diagnostic value.
