Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Widespread gene fusion artifacts in helminth genome annotations.

BMC genomics·2026
Same author

Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology.

Materials (Basel, Switzerland)·2025
Same author

More than Just Bread and Wine: Using Yeast to Understand Inherited Cytochrome Oxidase Deficiencies in Humans.

International journal of molecular sciences·2024
Same author

Novel <i>COX11</i> Mutations Associated with Mitochondrial Disorder: Functional Characterization in Patient Fibroblasts and <i>Saccharomyces cerevisiae</i>.

International journal of molecular sciences·2023
Same author

Phylogenomic Analysis of 155 Helminth Species Reveals Widespread Absence of Oxygen Metabolic Capacity.

Genome biology and evolution·2023
Same author

In-Vitro Study of Indium (III) Sulfate-Containing Medium on the Viability and Adhesion Behaviors of Human Dermal Fibroblast on Engineered Surfaces.

Materials (Basel, Switzerland)·2023

Related Experiment Video

Updated: Jun 10, 2026

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

Development of a microfluidic chip-based plasmid miniprep.

Victoria A Northrup1, Christopher J Backhouse, D Moira Glerum

  • 1Department of Medical Genetics, University of Alberta, Edmonton, Canada.

Analytical Biochemistry
|April 6, 2010
PubMed
Summary

This study introduces a rapid microfluidic chip-based plasmid DNA miniprep method. It significantly reduces processing and bacterial culture time, enabling faster molecular cloning and screening.

More Related Videos

Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 4, 2008

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jun 10, 2026

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 4, 2008

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Microfluidics

Background:

  • Plasmids are essential vectors in molecular cloning and genetic engineering.
  • Plasmid minipreps are crucial for isolating plasmid DNA from bacterial hosts like Escherichia coli.
  • Traditional miniprep protocols are time-consuming, often exceeding 16 hours.

Purpose of the Study:

  • To develop a faster, more efficient plasmid DNA miniprep method.
  • To reduce the time and resources required for plasmid isolation.
  • To enable rapid screening applications in molecular biology.

Main Methods:

  • Development of a microfluidic chip (MFC) for on-chip lysis and DNA trapping.
  • Utilized agarose for differential separation of plasmid DNA from bacterial chromosomal DNA.
  • Employed significantly fewer bacterial cells (10^5 times less) compared to conventional methods.

Main Results:

  • Achieved plasmid DNA isolation comparable in quality to traditional methods.
  • Reduced overall processing time, including bacterial culture, to within hours.
  • Demonstrated a substantial decrease in the number of Escherichia coli cells required.

Conclusions:

  • The MFC-based miniprep offers a rapid and efficient alternative to conventional protocols.
  • This technology is ideal for high-throughput screening and rapid molecular biology workflows.
  • Integration with on-chip transfection capabilities facilitates seamless plasmid manipulation on microfluidic platforms.