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Updated: Jun 14, 2026

Imaging Plasma Membrane Deformations With pTIRFM
Published on: April 2, 2014
Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
Devaraj Subramanian1, Vibor Laketa, Rainer Müller
1European Molecular Biology Institute, Cell Biology and Biophysics Unit, Heidelberg, Germany.
Abstract:
Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.
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