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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

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Nonlinear endomicroscopy using a double-clad fiber coupler.

Hongchun Bao¹, Seon Young Ryu, Byeong Ha Lee

¹Centre for Micro-Photonics, Faculty of Engineering & Industrial Sciences, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.

|April 6, 2010

Summary

This summary is machine-generated.

A novel double-clad fiber coupler enhances two-photon-excited fluorescence endomicroscopy by improving compactness and alignment stability. This new coupler achieves high excitation transmission and fluorescence collection efficiency, boosting imaging contrast.

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Area of Science:

Biomedical Optics
Optical Engineering
Microscopy

Background:

Two-photon-excited fluorescence endomicroscopy (TPEF) is a powerful in vivo imaging technique.
Traditional TPEF systems often rely on bulky dichroic mirrors for signal separation.
This limits miniaturization and alignment stability of endomicroscopes.

Purpose of the Study:

To develop and evaluate a double-clad fiber coupler for TPEF endomicroscopy.
To replace the dichroic mirror with a more compact and stable optical component.
To enhance the performance of TPEF endomicroscopes.

Main Methods:

A double-clad fiber coupler was designed and fabricated.
The coupler was integrated into a TPEF endomicroscope system.
Excitation laser transmission and fluorescence collection efficiency were measured.

Main Results:

The double-clad fiber coupler transmitted 62% of the excitation laser beam.
A fluorescence collection efficiency of 34% was achieved.
The endomicroscope demonstrated improved compactness and alignment stability.

Conclusions:

The developed double-clad fiber coupler effectively separates fluorescence from excitation light in TPEF endomicroscopy.
This component leads to more compact, stable, and higher-contrast endomicroscopy systems.
The achieved fluorescence collection efficiency is the highest reported for such couplers, enhancing imaging quality.