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Related Experiment Videos

Fluid-phase connectivity and translational diffusion in a eutectic, two-component, two-phase phosphatidylcholine

T Bultmann1, W L Vaz, E C Melo

  • 1Max-Planck-Institut für Biophysikalische Chemie, Göttingen, FRG.

Biochemistry
|June 4, 1991
PubMed
Summary

Fluorescence recovery after photobleaching (FRAP) reveals how lipid bilayer domain structures affect fluid-phase connectivity. Solid phases, like P beta

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Area of Science:

  • Membrane Biophysics
  • Physical Chemistry
  • Materials Science

Background:

  • Fluorescence recovery after photobleaching (FRAP) is a technique used to study molecular movement in lipid bilayers.
  • Previous studies demonstrated FRAP's utility in analyzing domain structures and fluid-phase connectivity in coexisting solid and fluid lipid phases.
  • Understanding these dynamics is crucial for comprehending membrane function and organization.

Purpose of the Study:

  • To investigate the translational diffusion and fluid-phase connectivity in lipid bilayers composed of binary mixtures of specific phosphatidylcholines.
  • To examine how different solid phases (Lc gel and P beta') influence fluid-phase connectivity.
  • To determine the impact of solid phase composition on the overall fluidity and domain structure of the lipid bilayer.

Main Methods:

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  • Utilized fluorescence recovery after photobleaching (FRAP) to monitor translational diffusion.
  • Employed a fluorescent lipid derivative, N-(7-nitrobenzoxa-2,3-diazol-4-yl) dilauroyl-phosphatidylethanolamine, for tracking molecular movement.
  • Examined lipid bilayers prepared from binary mixtures of 1-docosanoyl-2-dodecanoylphosphatidylcholine (C22:0C12:0PC) and 1,2-diheptadecanoylphosphatidylcholine (di-C17:0PC).

Main Results:

  • Observed distinct fluid-phase connectivity behaviors in lipid bilayers with coexisting solid and fluid phases.
  • Identified two key regions of temperature and composition where fluid and solid phases coexist.
  • In mixtures with <0.4 mole fraction di-C17:0PC, the fluid phase coexists with a mixed interdigitated Lc gel phase.
  • In mixtures with >0.4 mole fraction di-C17:0PC, the fluid phase coexists with a P beta' gel phase.

Conclusions:

  • Fluid-phase connectivity is significantly influenced by the type of coexisting solid phase.
  • When the solid phase is P beta' gel, fluid-phase connectivity is compromised, even with a small mass fraction of solid phase.
  • This suggests that the P beta' gel phase can effectively partition the fluid phase into disconnected domains, impacting membrane function.