Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compensating Random Transition-Detection Blackouts in Markov Networks.

Physical review letters·2026
Same author

Pedestrian's approach to large deviations in semi-Markov processes with an application to entropy production.

Physical review. E·2026
Same author

General theory for localizing the where and when of entropy production meets single-molecule experiments.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Estimator of entropy production for partially accessible Markov networks based on the observation of blurred transitions.

Physical review. E·2024
Same author

Nonequilibrium fluctuations of chemical reaction networks at criticality: The Schlögl model as paradigmatic case.

The Journal of chemical physics·2024
Same author

Time-Resolved Statistics of Snippets as General Framework for Model-Free Entropy Estimators.

Physical review letters·2023

Related Experiment Video

Updated: Jun 14, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Diffusing proteins on a fluctuating membrane: analytical theory and simulations.

Ellen Reister-Gottfried1, Stefan M Leitenberger, Udo Seifert

  • 1II Institut für Theoretische Physik, Universität Stuttgart, D-70550 Stuttgart, Germany.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|April 7, 2010
PubMed
Summary
This summary is machine-generated.

Membrane proteins diffuse slower than expected due to interactions with membrane shape. Analyzing membrane height correlations can determine protein diffusion coefficients.

More Related Videos

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Related Experiment Videos

Last Updated: Jun 14, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Area of Science:

  • Biophysics
  • Computational Biology

Background:

  • Membrane proteins are crucial for cellular functions.
  • Their lateral diffusion is influenced by membrane properties.

Purpose of the Study:

  • To investigate the impact of membrane protein-membrane interactions on lateral diffusion.
  • To develop analytical methods for calculating effective diffusion coefficients.

Main Methods:

  • Analytical calculations using a path-integral approach.
  • Computer simulations of membrane protein dynamics.
  • Analysis of correlation functions and membrane height spectra.

Main Results:

  • Protein-membrane interactions reduce effective protein mobility.
  • An analytical expression for the effective diffusion coefficient was derived.
  • Simulations confirmed analytical results with high accuracy.
  • Distinct alterations in membrane height correlation spectra were observed.

Conclusions:

  • Correlations between protein stochastic forces and membrane shape responses cause diffusion reduction.
  • Membrane height correlation decay offers a novel method to measure effective protein diffusion coefficients.