Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IL-1 production by human thymic dendritic cells: studies on the interrelation with DC accessory function.

M Lafontaine1, D Landry, N Blanc-Brunât

  • 1Département de Microbiologie et Immunologie, Université de Montréal, Québec, Canada.

Cellular Immunology
|July 1, 1991
PubMed
Summary

Human thymic dendritic cells (DCs) produce interleukin-1 (IL-1) upon stimulation, but IL-1 does not significantly enhance DC function in T cell development. Thymocyte contact can induce IL-1 production by DCs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reliability of CT Angiography in Cerebral Vasospasm: A Systematic Review of the Literature and an Inter- and Intraobserver Study.

AJNR. American journal of neuroradiology·2020
Same author

Assessment of baseline ecotoxicity of sediments from a prospective mining area enriched in light rare earth elements.

The Science of the total environment·2017
Same author

Relevance of omental pericellular adipose tissue collagen in the pathophysiology of human abdominal obesity and related cardiometabolic risk.

International journal of obesity (2005)·2016
Same author

The TNF-family cytokine TL1A promotes allergic immunopathology through group 2 innate lymphoid cells.

Mucosal immunology·2013
Same author

Moderators of psychological recovery from benign cancer screening results.

Current oncology (Toronto, Ont.)·2012
Same author

Dual vs. single encoding in recognition memory as a function of lag, delay, and availability of semantic information.

Memory & cognition·2011

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Thymic dendritic cells (DCs) are crucial for T lymphocyte generation.
  • Interleukin-1 (IL-1) is a key signal in T cell stimulation.

Purpose of the Study:

  • To investigate the IL-1 production capacity of human thymic DCs.
  • To determine the role of IL-1 in DC-mediated T cell accessory function.

Main Methods:

  • EL4/CTLL conversion assay to measure IL-1 activity.
  • Stimulation of DCs with lipopolysaccharide (LPS).
  • Neutralization assays using anti-IL-1 beta monoclonal antibodies (mAbs).

Main Results:

  • LPS-stimulated thymic DCs produce low levels of IL-1 compared to peripheral blood monocytes (PBM).

Related Experiment Videos

  • Thymic DC IL-1 production is not affected by indomethacin.
  • IL-1 beta is detected in DC-thymocyte co-cultures without mitogens, suggesting thymocyte contact induces IL-1 production.
  • IL-1 beta does not substitute for or enhance DC accessory function; anti-IL-1 beta mAbs do not block this function.

    • Conclusions:

    • Human thymic DCs are an intrathymic source of IL-1.
    • IL-1 beta is likely not a major factor in the accessory function of thymic DCs for mature thymocytes.
    • The precise role of IL-1 in thymocyte proliferation and maturation requires further investigation.