Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modular assembly of human auricular chondrocyte-derived microtissues for ear cartilage tissue engineering.

Acta biomaterialia·2026

Same author

Matrix-Enhanced Non-Viral Vectors: Aminated Collagen-Chitosan for Improved Gene Delivery.

ACS applied bio materials·2026

Same author

Quadruple immunotherapy with allogeneic natural killer cell infusion for recurrent neuroblastoma.

Cytotherapy·2026

Same author

Neurobehavioral outcomes and associated risk factors in pediatric brain tumor survivors.

Journal of neuro-oncology·2026

Same author

PINCH-1-dependent regulation on tumor matrix microenvironment in pancreatic cancer.

Biomaterials·2026

Same author

Efficacy and safety of marstacimab prophylaxis in hemophilia A/B with inhibitors: results from the phase 3 BASIS trial.

Blood·2025

Search research articles

Related Experiment Video

Updated: Jun 14, 2026

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

Mesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering.

Barbara Pui Chan¹, Ting Yan Hui, Mei Yi Wong

¹Medical Engineering Program, Department of Mechanical Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China. bpchan@hkucc.hku.hk

Tissue Engineering. Part C, Methods

|April 7, 2010

Summary

Injectable microspheres containing mesenchymal stem cells (MSCs) show promise as bone graft alternatives. These collagen-based microspheres demonstrate osteoconductivity, osteogenicity, and osteoinductivity for bone regeneration.

More Related Videos

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Related Experiment Videos

Last Updated: Jun 14, 2026

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Published on: June 1, 2012

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Published on: April 12, 2015

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Clinical demand for bone graft alternatives with superior osteoconductivity, osteoinductivity, and osteogenicity.
Previous development of injectable collagen-MSCs microspheres using microencapsulation technology.

Purpose of the Study:

To fabricate injectable microspheres with osteoconductivity, osteogenicity, and osteoinductivity.
To differentiate encapsulated mesenchymal stem cells (MSCs) towards osteogenic lineages within 3D collagen microspheres.

Main Methods:

Encapsulation of human or mouse bone marrow-derived MSCs within a biomimetic collagen fiber meshwork.
In vitro evaluation of microsphere osteogenicity, osteoconductivity, and osteoinductivity.
Assessment of osteogenic markers, calcium deposition, and co-culture studies.

Main Results:

Positive staining for osteogenic markers (alkaline phosphatase, calcium deposition) in differentiating MSCs.
Demonstrated osteoconductivity via calcium phosphate deposits and increased calcium content.
Confirmed osteoinductivity through the induction of osteogenic differentiation in undifferentiated MSCs.

Conclusions:

Injectable collagen-MSC microspheres can be fabricated with inherent osteoconductivity, osteogenicity, and osteoinductivity.
This technology offers a potential autologous cell-based injectable alternative for bone grafting applications.