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Updated: Jun 14, 2026

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

Selected factors affecting Staphylococcus aureus within silastic catheters.

Michelle J Henry-Stanley¹, Melody M Shepherd, Carol L Wells

¹Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.

The Journal of Surgical Research

|April 8, 2010

Summary

This summary is machine-generated.

Ethanol locking effectively sterilizes Staphylococcus aureus (S. aureus) contaminated catheters, preventing bacterial viability. However, bacterial remnants may persist on catheter surfaces after treatment.

Related Experiment Videos

Last Updated: Jun 14, 2026

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

Area of Science:

Microbiology
Infectious Diseases
Biomaterials

Background:

Catheter-related infections are common in hospitalized patients, often caused by Staphylococcus aureus.
Factors influencing S. aureus growth and survival within catheters are not well understood.

Purpose of the Study:

To investigate Staphylococcus aureus adherence to silastic catheters.
To evaluate the efficacy of ethanol locking in sterilizing S. aureus-contaminated catheters.

Main Methods:

In vitro assessment of S. aureus adherence to silastic catheters.
Evaluation of heparin, serum, and calcium effects on bacterial adherence.
Quantification of ethanol locking's impact on S. aureus viability in biofilms using scanning electron microscopy.

Main Results:

S. aureus demonstrated dose-dependent adherence to silastic catheters, unaffected by calcium or heparin.
Serum inhibited S. aureus adherence, while albumin did not.
Ethanol locking for 5 minutes to 24 hours eliminated detectable S. aureus viability in mature biofilms.

Conclusions:

Serum contains a component, not albumin, that inhibits S. aureus adherence to catheters.
Ethanol locking is an effective method for sterilizing S. aureus-contaminated catheters.
Ethanol locking eradicates viable bacteria but leaves nonviable bacterial structures on the catheter surface.