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Updated: Jun 14, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
Photoacoustic detection of H-D exchange in the system H(2)S-D(2)S
Abstract:
A method is described which allows a single-pulse analysis of photoacoustic signals. Isotope specific excitation of D(2)S and HDS is performed with a TEA CO(2) laser, and the concentration change of the two species caused by H-D exchange after fast mixing of H(2)S and D(2)S is monitored. No influence of the laser radiation on the exchange reaction could be detected. Thus the progress of an independent chemical reaction was analyzed by a concentration measurement. The time resolutions is determined by the repetition rate of the laser.
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