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Updated: Jun 14, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Untargeted metabolomics.

Nawaporn Vinayavekhin¹, Alan Saghatelian

¹Harvard University, Cambridge, Massachusetts, USA.

Current Protocols in Molecular Biology

|April 8, 2010

Summary

This summary is machine-generated.

Metabolomics reveals the diverse roles of metabolites in life, from energy storage to cell signaling. This study details mass spectrometry methods for discovering novel metabolites and pathways in bacterial systems.

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Last Updated: Jun 14, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Published on: May 20, 2013

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

Biochemistry
Analytical Chemistry
Microbiology

Background:

Metabolites are crucial molecules involved in primary metabolic processes and secondary functions like cell signaling and virulence.
Understanding the in vivo roles of metabolites is essential for advancing biological knowledge.
Current methods for metabolite analysis can be accelerated to enhance the discovery of novel metabolites and pathways.

Purpose of the Study:

To detail the steps for performing a mass spectrometry (MS)-based untargeted metabolomics experiment using bacterial supernatants.
To provide protocols for analyzing hydrophobic metabolites.
To demonstrate the structural characterization of a novel metabolite.

Main Methods:

Untargeted metabolomics using mass spectrometry (MS) to analyze all detectable ions within a specific mass range.
Protocols specifically designed for the analysis of hydrophobic metabolites in bacterial supernatants.
Methodology for the structural characterization of newly identified metabolites.

Main Results:

The study provides a comprehensive protocol for untargeted metabolomics in bacterial systems.
The described methods enable the detection and analysis of hydrophobic metabolites.
A practical example of structurally characterizing a novel metabolite is presented.

Conclusions:

Untargeted metabolomics is a powerful tool for exploring the metabolome and discovering novel compounds.
The developed protocols facilitate the comprehensive analysis of metabolites, including hydrophobic ones.
This approach advances our understanding of metabolite functions and metabolic pathways in bacteria.