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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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Published on: April 6, 2022

Oxygen mass transfer in a human tissue-engineered trachea.

Efrem Curcio¹, Paolo Macchiarini, Loredana De Bartolo

¹Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci cubo 17/C, I-87030 Rende (CS), Italy.

|April 10, 2010

Summary

Computational modeling revealed oxygen transport limitations in engineered trachea grafts, explaining stem cell migration. This finding is crucial for improving tissue-engineered airway therapies.

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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

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Published on: January 6, 2010

Area of Science:

Biomedical Engineering
Regenerative Medicine
Computational Biology

Background:

First human tissue-engineered trachea replacement utilized decellularized donor trachea seeded with recipient cells.
Postoperative analysis revealed stem cell migration within the graft, prompting investigation into underlying mechanisms.

Purpose of the Study:

To develop a computational model of oxygen transport in a tissue-engineered trachea construct.
To investigate the hypothesis that oxygen gradients drive cellular migration within the graft.

Main Methods:

Finite element method (FEM) was employed to solve the computational model.
Oxygen transport dynamics were simulated within the engineered trachea construct.

Main Results:

Computational results confirmed oxygen transport limitations, particularly in the proximal section (first 2.8 cm).
Severe oxygen mass transfer limitation was observed within 650 micrometers in the proximal region.
At high cell density, 30% of the section area experienced oxygen deficiency (partial pressure < 38 mmHg).
Thiele modulus values indicated significant mass transfer limitations in the chondrocyte compartment.

Conclusions:

Oxygen transport limitations are a critical factor influencing cell behavior in engineered trachea.
The proximal region of the graft presents the most significant challenge for oxygen diffusion.
Findings provide a rationale for observed stem cell migration and inform future graft design for improved vascularization and cell survival.