Related Experiment Video
Updated: Jun 14, 2026
09:01
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
New steroidal saponin from Hosta sieboldiana
Hiroshi Yada1, Toshiyuki Kimura, Masahiro Suzuki
1National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. yada33@affrc.go.jp
Bioscience, Biotechnology, and Biochemistry
|April 10, 2010
Summary
Hosta sieboldiana extract inhibited mouse leukemia cell growth. A novel steroidal saponin with a complex glycoside structure was identified from the plant.
Area of Science:
- Phytochemistry
- Pharmacology
- Natural Products Chemistry
Background:
- Hosta sieboldiana is an edible plant with potential medicinal properties.
- Leukemic cell lines are commonly used in preclinical cancer research.
Purpose of the Study:
- To investigate the cytotoxic effects of Hosta sieboldiana extract on leukemic cells.
- To isolate and characterize novel compounds from Hosta sieboldiana.
Main Methods:
- Extraction and isolation of compounds from Hosta sieboldiana.
- Spectroscopic analysis (NMR, MS) for structure elucidation.
- Cytotoxicity assays using P388 mouse leukemic cells.
Main Results:
- The extract of Hosta sieboldiana demonstrated significant suppression of P388 mouse leukemic cell growth.
- A new steroidal saponin, (25R)-3beta-(alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranosyl)-5alpha-spirostan-2alpha-ol, was isolated and characterized.
Conclusions:
- Hosta sieboldiana possesses compounds with anticancer potential against leukemia.
- The identified steroidal saponin is a novel chemical entity with potential for further pharmacological investigation.
