Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid antimicrobial susceptibility testing directly from positive blood cultures.

The Analyst·2026
Same author

Segmentation of single-cell impedance signals using deep learning: a multi-dataset study.

IEEE transactions on bio-medical engineering·2026
Same author

Air pollution and alveolar health.

European respiratory review : an official journal of the European Respiratory Society·2025
Same author

Trapping of Micro- and Nanoparticles within Microfluidic Constrictions in AC Electric Fields.

Analytical chemistry·2025
Same author

Single-cell impedance spectroscopy of nucleated cells.

Lab on a chip·2025
Same author

Copper-enriched automotive brake wear particles perturb human alveolar cellular homeostasis.

Particle and fibre toxicology·2025
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2026

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
10:51

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Published on: September 26, 2017

On-chip epithelial barrier function assays using electrical impedance spectroscopy.

Tao Sun1, Emily J Swindle, Jane E Collins

  • 1Nano Research Group, School of Electronics and Computer Science, University of Southampton, SO17 1BJ, United Kingdom. ts5@ecs.soton.ac.uk

Lab on a Chip
|April 10, 2010
PubMed
Summary
This summary is machine-generated.

A novel bio-impedance chip effectively monitors airway epithelial cell function in real-time. This technology accurately measures disruptions in epithelial barrier integrity caused by common agents.

More Related Videos

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
12:30

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines
09:38

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Published on: September 22, 2023

Related Experiment Videos

Last Updated: Jun 14, 2026

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
10:51

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Published on: September 26, 2017

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
12:30

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines
09:38

Measuring Changes in Brain Endothelial Barrier Integrity with Two Impedance-based Biosensors in Response to Cancer Cells and Cytokines

Published on: September 22, 2023

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Materials Science

Background:

  • Epithelial cell monolayers are crucial models for studying airway epithelium.
  • Real-time monitoring of epithelial barrier function is essential for understanding cellular responses.
  • Traditional methods for assessing epithelial integrity can be time-consuming and less dynamic.

Purpose of the Study:

  • To develop and validate a bio-impedance chip for real-time kinetic monitoring of epithelial cell monolayers.
  • To assess the chip's ability to detect changes in epithelial barrier function.
  • To compare the bio-impedance chip's performance with conventional trans-epithelial electrical resistance measurements.

Main Methods:

  • Electrochemical deposition of polypyrrole (PPy) doped with polystyrene sulfonate (PSS) onto gold electrodes.
  • Culture of human bronchial epithelial cells (16-HBE 14o-) in Transwells.
  • Impedance spectroscopy combined with finite element and equivalent circuit modeling.
  • Treatment of cell monolayers with Triton X-100 and ethylene glycol-bis(2-aminoethyl-ether)-N,N,N'N'-tetraacetic acid (EGTA).
  • Immunofluorescent staining for ZO-1 tight junction protein.

Main Results:

  • The bio-impedance chip successfully measured real-time changes in epithelial barrier function upon treatment with Triton X-100 and EGTA.
  • Impedance data correlated well with conventional trans-epithelial electrical resistance measurements.
  • EGTA treatment was confirmed to disrupt tight junctions via ZO-1 staining.

Conclusions:

  • The developed bio-impedance chip provides a sensitive and reliable method for real-time monitoring of epithelial cell monolayer kinetics.
  • This technology offers a valuable tool for in vitro studies of airway epithelium and drug screening.
  • The chip's ability to detect barrier disruption enhances its utility in physiological and pathological research.