Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Personal-computer-based system for electron beam X-ray microanalysis of biological samples.

M C Foster1, A J Saubermann

  • 1Department of Anaesthesiology, SUNY, Stony Brook 11794-8480.

Journal of Microscopy
|February 1, 1991
PubMed
Summary

A new personal computer system offers an affordable method for quantitative elemental analysis in electron microscopy labs. This system enables detailed analysis of cryosectioned biological samples, making advanced techniques more accessible.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baseline neutrophil-to-lymphocyte ratio as a predictive and prognostic biomarker in patients with metastatic castration-resistant prostate cancer treated with cabazitaxel versus abiraterone or enzalutamide in the CARD study.

ESMO open·2021
Same author

Filtration Markers, Cardiovascular Disease, Mortality, and Kidney Outcomes in Stable Kidney Transplant Recipients: The FAVORIT Trial.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
Same author

Estimated GFR for Living Kidney Donor Evaluation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
Same author

A review of 155 extra-anatomic bypass grafts.

Annals of the Royal College of Surgeons of England·2009
Same author

A new short-acting non-depolarizing muscle relaxant (SZ1677) without cardiovascular side-effects.

Acta anaesthesiologica Scandinavica·2003
Same author

Vaginal bleeding as a presentation of metastatic renal cell carcinoma.

BJU international·1999

Area of Science:

  • Materials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Electron microscopy is crucial for biological sample analysis.
  • Quantitative elemental analysis provides vital information about sample composition.
  • Existing systems can be expensive and complex.

Purpose of the Study:

  • To develop an affordable quantitative elemental analysis system for electron microscopy.
  • To demonstrate the feasibility of using personal computers for X-ray analysis.
  • To create a user-friendly interface for data acquisition and analysis.

Main Methods:

  • Assembled hardware and software to control scanning electron microscope beam.
  • Developed software for X-ray spectra collection, analysis, and data management.

Related Experiment Videos

  • Integrated personal computer components with commercial hardware and software.

    • Main Results:

    • Created a menu-driven interface for spectra acquisition and analysis.
    • Enabled spot analyses, video imaging, and quantitative elemental imaging.
    • Calculated wet and dry weight concentrations from hydrated and freeze-dried samples.
    • Facilitated data transfer in ASCII format and image printing.

    Conclusions:

    • A personal computer-based system is a feasible and cost-effective solution for quantitative elemental analysis in electron microscopy.
    • The developed system enhances accessibility to advanced analytical techniques for biological samples.
    • The system provides comprehensive data, including elemental composition and imaging, supporting detailed biological research.