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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Label-free capacitive biosensor for sensitive detection of multiple biomarkers using gold interdigitated capacitor
Anjum Qureshi1, Javed H Niazi, Saravan Kallempudi
1Faculty of Engineering & Natural Sciences, Sabanci University, Orhanli, Tuzla 34956, Istanbul, Turkey.
Biosensors & Bioelectronics
|April 13, 2010
Summary
A novel capacitive immunosensor detects inflammation and cardiovascular risk biomarkers like C-reactive protein (CRP), TNFalpha, and IL6. This label-free, multianalyte biosensor offers sensitive detection for early disease diagnosis.
Area of Science:
- Biomedical Engineering
- Biosensor Technology
- Immunosensing
Background:
- Inflammation markers such as C-reactive protein (CRP), TNFalpha, and IL6 are linked to cardiovascular risk (CVR).
- Early detection of disease biomarkers is crucial for accurate risk prediction and timely intervention.
- Existing detection methods may lack the sensitivity or multiplexing capability for comprehensive disease risk assessment.
Purpose of the Study:
- To develop a highly sensitive, label-free, multianalyte capacitive immunosensor for detecting CRP, TNFalpha, and IL6.
- To evaluate two distinct lab-on-a-chip formats for simultaneous biomarker detection using gold interdigitated electrodes (GID).
- To assess the clinical potential of the developed biosensor for early diagnosis of inflammatory and CVR-related conditions.
Main Methods:
- Fabrication of capacitive immunosensors using gold interdigitated electrodes (GID) capacitor arrays.
- Immobilization of specific antibodies (anti-CRP, anti-TNFalpha, anti-IL6) onto GID arrays in distinct formats.
- Detection of biomarkers based on changes in capacitive/dielectric properties.
- Comparison of two formats: Format I (separate antibody immobilization per capacitor) and Format II (co-immobilized antibody mixtures).
Main Results:
- Format I demonstrated detection ranges of 25 pg/ml to 25 ng/ml for CRP and IL6, and 25 pg/ml to 1 ng/ml for TNFalpha.
- Format II, utilizing co-immobilized antibodies, achieved sensitive detection for all three biomarkers within the 25 pg/ml to 25 ng/ml range.
- Both formats showed reliable detection of target biomarkers, with Format II offering efficient simultaneous detection.
Conclusions:
- The developed capacitive immunosensor is highly sensitive and label-free, enabling multianalyte detection.
- The biosensor shows significant clinical value for detecting panels of inflammation and CVR markers.
- This technology holds great potential for the early diagnosis of diseases by analyzing biomarker panels in suspected individuals.
