Related Experiment Video

Updated: May 14, 2026

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11:10

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Published on: November 16, 2011

Carcinoembryonic antigen-related cell adhesion molecule 2 controls energy balance and peripheral insulin action in

Garrett Heinrich1, Sumona Ghosh, Anthony M Deangelis

  • 1The Center for Diabetes and Endocrine Research, Department of Physiology & Pharmacology, College of Medicine, University of Toledo, Health Science Campus, Toledo, Ohio 43614, USA.

Gastroenterology
|April 13, 2010
PubMed
Abstract

Insights

Carcinoembryonic antigen-related cell adhesion molecule 2 (CEACAM2) deficiency in mice causes obesity due to increased appetite and lower energy use. This highlights CEACAM2

Area of Science:

  • Endocrinology
  • Metabolism
  • Molecular Biology

Background:

  • Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) influences hepatic insulin clearance.
  • CEACAM2 is a related protein with a specific tissue distribution.
  • The physiological roles of CEACAM2 remain largely uncharacterized.

Purpose of the Study:

  • To investigate the function of CEACAM2 in regulating energy balance and insulin sensitivity.
  • To characterize the phenotype of CEACAM2-deficient mice.

Main Methods:

  • Generation of Ceacam2-null (Cc2-/-) mice.
  • Phenotypic characterization including hyperinsulinemic-euglycemic clamp analysis, indirect calorimetry, and body composition analysis.
  • Pair feeding and insulin tolerance tests were performed.

Main Results:

  • Female Cc2-/- mice developed obesity, hyperphagia, and reduced energy expenditure.
  • Hyperphagia contributed to peripheral insulin resistance, particularly in skeletal muscle.
  • Impaired fatty acid oxidation and glucose disposal were observed in skeletal muscle.
  • Increased hypothalamic fatty acid synthase and hyperinsulinemia were implicated in hyperphagia.

Conclusions:

  • CEACAM2 is expressed in the mouse hypothalamus.
  • CEACAM2 plays a crucial role in regulating energy balance and insulin sensitivity.
  • CEACAM2 deficiency leads to obesity through hyperphagia and altered energy expenditure.

More Related Videos

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice10:28

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Published on: May 24, 2018

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
Type II Diabetes I: Introduction01:26

Type II Diabetes I: Introduction

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin resistance, in which target tissues such as the liver, muscle, and adipose tissue respond poorly to insulin. It is also associated with inadequate compensatory insulin secretion, where pancreatic β-cells fail to produce sufficient insulin. Together, these abnormalities lead to persistent hyperglycemia.EtiologyT2DM develops through a complex interaction of genetic predisposition and environmental or...
Type II Diabetes II: Pathophysiology01:24

Type II Diabetes II: Pathophysiology

PathophysiologyType 2 diabetes mellitus (T2DM ) is a chronic metabolic disorder characterized by insulin resistance and progressive pancreatic β-cell dysfunction, leading to impaired glucose homeostasis. It results from interactions among genetic predisposition, environmental factors, and metabolic stressors, such as overnutrition and a sedentary lifestyle.Insulin Resistance and Glucose DysregulationEarly T2DM involves insulin resistance in skeletal muscle, adipose tissue, and the liver.