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Updated: Jun 14, 2026

Studying Muscle Transcriptional Dynamics at Single-molecule Scales in Drosophila
Published on: September 8, 2023
In situ detection and genotyping of individual mRNA molecules
Chatarina Larsson1, Ida Grundberg, Ola Söderberg
1Department of Genetics and Pathology, Uppsala University, Sweden.
Abstract:
Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
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