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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 13, 2026

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging
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Published on: February 3, 2015

Hybrid PET-optical imaging using targeted probes.

Matthias Nahrendorf1, Edmund Keliher, Brett Marinelli

  • 1Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. MNahrendorf@mgh.harvard.edu

Proceedings of the National Academy of Sciences of the United States of America
|April 14, 2010
PubMed
Summary

Fluorescence-mediated tomography (FMT) precisely matches radionuclide imaging (PET) for molecular imaging probes. This optical method can guide the development of new nuclear imaging agents for cancer research.

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In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Published on: November 11, 2017

Area of Science:

  • Molecular imaging
  • Biomedical optics
  • Nuclear medicine
  • Nanotechnology

Background:

  • Multimodal fusion imaging combining Positron Emission Tomography (PET) and X-ray Computed Tomography (CT) is a well-established technique.
  • There is a need for complementary imaging modalities that can aid in the development and screening of molecular imaging probes.

Purpose of the Study:

  • To demonstrate the congruence between fluorescence-mediated tomography (FMT) and PET for molecular imaging.
  • To evaluate the potential of FMT as a surrogate for PET in the screening and development of radionuclide-based imaging agents.

Main Methods:

  • Development of biocompatible nanoparticles containing both a (18)F isotope for PET and a far-red fluorochrome for FMT.
  • Correlation analysis of probe concentration and spatial signal distribution between FMT and PET measurements.
  • In vivo imaging in a mouse cancer model using different probes to simultaneously assess tumoral proteases, macrophage content, and integrin expression.

Main Results:

  • Excellent correlation (r(2) > 0.99) between FMT and PET for probe concentration.
  • High correlation (r(2) > 0.85) between FMT and PET for spatial signal distribution.
  • Demonstration of distinct in vivo tumoral localization of multiple imaging probes using multi-channel FMT.

Conclusions:

  • FMT exhibits high congruence with PET imaging, enabling seamless integration and visualization of molecular and structural data.
  • FMT can serve as a valuable surrogate modality for the screening and development of novel radionuclide-based imaging agents.
  • This approach facilitates simultaneous assessment of multiple biological targets in vivo, advancing preclinical cancer research.