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Updated: Jun 13, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Primer-free aptamer selection using a random DNA library.

Weihua Pan1, Gary A Clawson

  • 1Gittlen Cancer Research Foundation, Hershey Medical Center, Hershey, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 14, 2010
PubMed
Summary
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A novel primer-free method simplifies aptamer selection by eliminating interfering sequences. This approach enhances the efficiency and accuracy of identifying high-affinity DNA aptamers for various applications.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nucleic Acid Chemistry

Background:

  • Aptamers are oligonucleotide molecules with high affinity and specificity, comparable to antibodies.
  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is the standard method for aptamer selection.
  • Traditional SELEX relies on fixed primer sequences that can introduce errors, complicating the selection process.

Purpose of the Study:

  • To develop a modified SELEX protocol that removes the requirement for fixed primer sequences.
  • To improve the accuracy and efficiency of aptamer selection by eliminating primer-related interferences.
  • To present a novel, primer-free approach for generating high-affinity DNA aptamers.

Main Methods:

  • Development of a primer-free Systematic Evolution of Ligands by Exponential Enrichment (SELEX) protocol.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

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Last Updated: Jun 13, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

  • Utilized a random DNA library without flanking primer binding sites.
  • Performed multiple rounds of selection and amplification without primer regeneration steps.

    • Main Results:

    • Successfully demonstrated a functional primer-free SELEX protocol.
    • The new method avoids issues associated with fixed primer sequences, such as false positives and negatives.
    • This approach facilitates the selection of high-affinity aptamers with improved specificity.

    Conclusions:

    • The primer-free SELEX protocol offers a significant advancement over traditional methods.
    • This method streamlines aptamer discovery and enhances its reliability.
    • Primer-free aptamer selection holds promise for various diagnostic and therapeutic applications.