[Investigation of bacterial translocation regularities in diffuse peritonitis using labeled radionuclide...

Area of Science:

• Microbiology
• Pathophysiology
• Surgical Science

Background:

• The intestinal barrier normally prevents bacterial translocation.
• Diffuse peritonitis compromises the integrity of the intestinal barrier.
• Opportunistic pathogenic microflora can translocate from the gut and abdominal cavity.

Purpose of the Study:

• To investigate the regularities of bacterial translocation in diffuse peritonitis.
• To understand the role of the intestinal barrier in peritonitis.
• To assess the impact of surgical interventions on bacterial translocation.

Main Methods:

• Utilized labeled radionuclide Escherichia coli (colibacillus) for tracking bacterial movement.
• Studied bacterial translocation from the small intestine lumen and abdominal cavity.
• Observed translocation dynamics in a diffuse peritonitis model.
• Evaluated effects of relaparotomy and intestinal manipulation.

Main Results:

• The intestinal barrier is impermeable to bacteria under normal conditions.
• Bacterial translocation and peritoneal resorption begin within minutes of diffuse peritonitis onset.
• The primary source of bacterial toxemia shifts from the peritoneum to the intestine during peritonitis progression.
• Relaparotomy significantly activates bacterial translocation into portal blood flow and systemic circulation.

Conclusions:

• Diffuse peritonitis rapidly compromises the intestinal barrier, leading to bacterial translocation.
• The intestinal barrier's integrity is crucial for preventing systemic bacterial spread in peritonitis.
• Surgical interventions like relaparotomy can worsen bacterial translocation, necessitating careful management.