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Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Umbilical cord stem cell seeding on fast-resorbable calcium phosphate bone cement
Hockin H K Xu1, Liang Zhao, Michael S Detamore
1Department of Endodontics, Prosthodontics, and Operative Dentistry, University of Maryland Dental School, Baltimore, Maryland 21201, USA. hxu@umaryland.edu
Tissue Engineering. Part A
|April 15, 2010
Summary
Human umbilical cord mesenchymal stem cells (hUCMSCs) show excellent proliferation and bone-forming potential on fast-resorbing calcium phosphate cement (CPC) scaffolds. This offers a promising alternative for bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Stem Cell Biology
Background:
- Tissue engineering holds significant potential for bone regeneration.
- Human umbilical cord mesenchymal stem cells (hUCMSCs) offer a less invasive source compared to bone marrow stem cells.
- Calcium phosphate cement (CPC) is a key material in bone regeneration scaffolds.
Purpose of the Study:
- To evaluate the physical properties of novel CPC scaffolds with enhanced dissolution/resorption rates.
- To assess the attachment, proliferation, and osteogenic differentiation of hUCMSCs on these modified CPC scaffolds.
Main Methods:
- CPC scaffolds were fabricated with varying ratios of tetracalcium phosphate and dicalcium phosphate anhydrous.
- Dissolution rates, mechanical strength, and modulus were measured.
- hUCMSCs were cultured on the scaffolds, and their proliferation and osteogenic differentiation markers (alkaline phosphatase, osteocalcin, collagen I, osterix) were analyzed.
Main Results:
- CPC scaffolds with a 1/3 ratio exhibited a 40% faster dissolution rate than controls, with comparable strength and modulus exceeding cancellous bone values.
- hUCMSCs demonstrated robust attachment and rapid proliferation on the nano-apatitic CPC surfaces.
- Significant upregulation of osteogenic gene expression (alkaline phosphatase, osteocalcin, collagen I, osterix) was observed in hUCMSCs.
Conclusions:
- hUCMSCs exhibit excellent proliferation and osteogenic differentiation on CPC scaffolds with enhanced dissolution/resorption rates.
- High-strength, fast-resorbing CPC scaffolds support hUCMSC osteogenesis, presenting a viable alternative to bone marrow stem cells for bone tissue engineering.
- These findings have broad implications for advancing stem cell-based bone regeneration strategies.
