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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jun 13, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

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Gabor-based fusion technique for Optical Coherence Microscopy.

Jannick P Rolland1, Panomsak Meemon, Supraja Murali

  • 1The Institute of Optics, University of Rochester, Rochester, NY 14627, USA. rolland@optics.rochester.edu

Optics Express
|April 15, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces an advanced optical coherence microscopy technique for high-resolution imaging. It achieves subcellular resolution across the entire sample depth without mechanical scanning or adaptive optics.

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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Area of Science:

  • Biomedical Optics
  • Microscopy Techniques
  • Optical Engineering

Background:

  • Traditional optical coherence microscopy (OCM) often requires mechanical scanning or adaptive optics for high-resolution depth imaging.
  • Achieving invariant lateral resolution across a large field of view presents a significant challenge in microscopy.

Purpose of the Study:

  • To present an image acquisition method using a novel liquid-lens-based dynamic focusing optical probe.
  • To demonstrate an automatic data fusion technique for producing in-focus, high-resolution images throughout a sample's depth.
  • To showcase the capability of this integrated system for in vivo imaging.

Main Methods:

  • Utilized a previously reported liquid-lens-based dynamic focusing optical probe with 2-micrometer invariant lateral resolution.
  • Developed and applied an automatic Gabor-based data fusion method for image reconstruction.
  • Performed imaging on African frog tadpole (Xenopus laevis) and human skin samples.

Main Results:

  • Achieved subcellular resolution (0.5 mm lateral x 0.5 mm axial) without x-y translation stages, depth scanning, adaptive optics, or manual intervention.
  • Demonstrated high-resolution imaging across the full imaging depth of the sample.
  • Acquired in vivo images of both biological specimens and human skin.

Conclusions:

  • The combined optical probe and data fusion method enable efficient, high-resolution, depth-invariant imaging.
  • This technique significantly simplifies the OCM setup by eliminating the need for complex hardware.
  • The system shows great potential for label-free in vivo imaging applications in biology and medicine.