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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

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Published on: October 1, 2014

Optofluidic microscope with 3D spatial resolution.

Asger Laurburg Vig¹, Rodolphe Marie, Eric Jensen

¹DTU Nanotech, Department of Micro and Nanotechnology, Technical University of Denmark, Building 345east, Ørsteds Plads, DK-2800 Kongens Lyngby, Denmark.

|April 15, 2010

Summary

This summary is machine-generated.

This study integrates an optofluidic microscope (OFM) into a microfluidic pinched flow fractionation (PFF) device for precise 3D particle analysis. The system accurately measures particle size, position, and velocity, advancing microfluidic separation techniques.

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Area of Science:

Microfluidics
Optical Microscopy
Biotechnology

Background:

Microfluidic devices are crucial for particle separation and analysis.
Accurate 3D spatial resolution and velocity measurements are challenging in microfluidic systems.
Conventional methods often lack the precision required for detailed particle characterization.

Purpose of the Study:

To develop an on-chip optical detection system with 3D spatial resolution.
To integrate an optofluidic microscope (OFM) with a microfluidic pinched flow fractionation (PFF) device.
To enable on-chip particle image velocimetry (PIV) for enhanced microfluidic analysis.

Main Methods:

Integration of an optofluidic microscope (OFM) into a microfluidic pinched flow fractionation (PFF) device.
Utilized on-chip particle image velocimetry (PIV) for velocity measurements.
Determined position and velocity of fluorescently labeled polystyrene microspheres (1-4 µm) using OFM.

Main Results:

OFM achieved high accuracy in size-separated microsphere detection (
Accurate measurements of microsphere position (1.4 µm) and velocity (0.08 mm/s) in 3D.
Observed microspheres migrating towards the channel center in height distribution.

Conclusions:

The integrated OFM-PFF system provides precise 3D optical detection and PIV capabilities on-chip.
This technology offers a significant advancement for high-resolution analysis within microfluidic separation devices.
Demonstrated the system's effectiveness for characterizing microsphere behavior during fractionation.