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Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
Published on: January 31, 2020
[Dynamic experiment on controlling of Microcystis aeruginosa by UV-C irradiation]
Kan Yuan1, Xian-zhong Mao, Yi Tao
1Research Center for Environmental Engineering and Management, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. hitless@gmail.com
Abstract:
A plug-flow UV-C reactor equipped with low pressure UV lamp was utilized to study the suppression effect after UV-C irradiation under the dynamic conditions on Microcystis aeruginosa, a typical cyanobacterium in algae blooms in China. The culture fluid of Microcystis aeruginosa was exposed to UV-C irradiation when pumped through the reactor. After that, the fluid was incubated under the normal culture condition, and sampled at 2 h, 1 d, 3 d, 5 d, 7 d, 9 d for determination of cell density using the inverted system fluorescence microscope. The experiments showed that, UV-C irradiation did not cause severe cell lysis, and UV-C irradiation at dose ranged from 36 to 115 mW x s x cm(-2), and 31 to 50 mW x s x cm(-2) could suppress Microcystis aeruginosa biomass growth for the 2.6 x 10(5)-2.7 x 10(5) cells x mL(-1) and 9.0 x 10(5)-1.15 x 10(6) cells x mL(-1) fluid in 9 days, respectively.
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