Related Experiment Video
Updated: Jun 13, 2026

Scanning Electron Microscopic Evaluation of Surface Defects of Remover Retreatment File After Single and Multiple Uses
Published on: October 11, 2024
Dental burs and endodontic files: are routine sterilization procedures effective?
Archie Morrison1, Susan Conrod
1Department of Oral and Maxillofacial Surgery, Dalhousie University, Halifax, Nova Scotia.
New dental burs and endodontic files are not sterile. Current sterilization methods are inadequate for used instruments, suggesting they may need to be single-use devices.
Area of Science:
- Dental instruments
- Sterilization protocols
- Infection control
Background:
- Dental burs and endodontic files possess complex miniature architectures, complicating precleaning and sterilization.
- Current sterilization protocols for these instruments require careful consideration, with some advocating for single-use device designation.
Purpose of the Study:
- To evaluate the efficacy of various dental sterilization techniques for endodontic files and dental burs.
- To determine if new dental burs and endodontic files are sterile upon manufacturer packaging.
Main Methods:
- Assessed sterility of new and used dental burs and endodontic files before and after sterilization.
- Tested new instruments directly from packaging and after sterilization.
- Precleaned, sterilized, and tested used instruments for pathogens using bacterial growth observation in Todd-Hewitt broth.
Main Results:
- Sterilization was 100% effective for unused burs and files.
- Sterilization was less than 100% effective for used instruments.
- Contamination rates post-sterilization ranged from 15% for used burs to 58% for used files.
Conclusions:
- Dental burs and endodontic files from manufacturers are not sterile and require sterilization before initial use.
- Tested resterilization procedures were inadequate for used instruments.
- More effective sterilization methods are needed, or these instruments should be considered single-use.
Related Concept Videos
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Hand hygiene
Hand washing...
Key Techniques in Microbiology
