Related Experiment Video
Updated: Jun 25, 2026

Scanning Electron Microscopic Evaluation of Surface Defects of Remover Retreatment File After Single and Multiple Uses
Published on: October 11, 2024
Dental burs and endodontic files: are routine sterilization procedures effective?
Archie Morrison1, Susan Conrod
1Dalhousie University, Halifax, Nova Scotia.
New dental instruments are not sterile and require sterilization before first use. Current sterilization methods are inadequate for used burs and endodontic files, suggesting they may need to be single-use devices.
Area of Science:
- Dental instruments
- Sterilization protocols
- Infection control
Background:
- Dental burs and endodontic files have complex designs that hinder effective precleaning and sterilization.
- Current sterilization protocols for these instruments require careful consideration.
- The possibility of classifying these instruments as single-use devices has been raised.
Purpose of the Study:
- To evaluate the efficacy of current dental sterilization techniques for endodontic files and dental burs.
- To determine if new dental burs and endodontic files are sterile upon arrival from manufacturers.
Main Methods:
- Assessed the sterility of new and used dental burs and endodontic files.
- Tested new instruments directly from packaging, with and without sterilization.
- Precleaned, packaged, and resterilized used instruments before pathogen testing.
- Incubated individual items in Todd-Hewitt broth for 72 hours to detect bacterial growth.
Main Results:
- Sterilization was 100% effective for new, unused burs and files.
- Sterilization was less than 100% effective for used instruments.
- Contamination rates after sterilization ranged from 15% for used burs to 58% for used files.
Conclusions:
- Dental burs and endodontic files from manufacturers are not sterile and require sterilization before initial use.
- The tested resterilization procedures were insufficient for used instruments.
- More effective sterilization methods are needed, or these instruments should be considered for single-use applications.
Related Concept Videos
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Key Techniques in Microbiology
Antimicrobial Effectiveness
