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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

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Protein biotinylation.

Giuliano Elia¹

¹University College Dublin, Dublin, Ireland.

Current Protocols in Protein Science

|April 16, 2010

Summary

Biotinylation protocols enable versatile labeling of proteins for biotechnology. These methods facilitate applications in vitro, in vivo, and ex vivo, expanding research capabilities.

Area of Science:

Biotechnology
Molecular Biology
Biochemistry

Background:

The high-affinity interaction between biotin and avidin is a cornerstone of molecular biology techniques.
Avidin and its homologs form exceptionally strong noncovalent bonds with biotin (vitamin H).

Purpose of the Study:

To provide fundamental protocols for biotinylation of proteins.
To detail methods applicable across various experimental settings, including in vitro, in vivo, and ex vivo labeling.

Main Methods:

Describes basic protocols for conjugating biotin to diverse protein reactive groups.
Outlines procedures for labeling proteins in solution, within tissue culture, and in whole organisms.

Main Results:

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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

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Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

Demonstrates the utility of biotinylation for a wide range of biotechnological applications.

Provides adaptable methods for labeling proteins in vitro, in tissue culture, in vivo, and ex vivo.

Conclusions:

The described biotinylation protocols offer versatile tools for researchers in biotechnology.
These methods enhance the ability to label and detect biomolecules across different biological contexts.