Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

33.4K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
33.4K
Cell-surface Signaling01:21

Cell-surface Signaling

54.4K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.4K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

27.4K
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
27.4K
Surface Tension and Surface Energy01:16

Surface Tension and Surface Energy

3.3K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
3.3K
Protein and Protein Structure02:15

Protein and Protein Structure

87.6K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
87.6K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intratumoral administration of daromun in non-melanoma skin cancer: Preliminary results from a phase 2 non-randomized controlled trial.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

DIGE Analysis of Animal Tissues.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Citrullination of Proteins as a Specific Response Mechanism in Plants.

Frontiers in plant science·2021
Same author

Stereotactic ablative body radiotherapy (SABR) combined with immunotherapy (L19-IL2) versus standard of care in stage IV NSCLC patients, ImmunoSABR: a multicentre, randomised controlled open-label phase II trial.

BMC cancer·2020
Same author

A phase II study of the L19IL2 immunocytokine in combination with dacarbazine in advanced metastatic melanoma patients.

Cancer immunology, immunotherapy : CII·2019
Same author

DIGE Analysis of Animal Tissues.

Methods in molecular biology (Clifton, N.J.)·2017

Related Experiment Video

Updated: Feb 2, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
12:03

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Published on: July 23, 2017

9.9K

Cell Surface Protein Biotinylation for SDS-PAGE Analysis.

Giuliano Elia1

  • 1Philochem AG, Otelfingen, Switzerland. giuliano.elia@philogen.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2018
PubMed
Summary

This chapter details cell surface protein purification using biotinylation and avidin affinity chromatography. These methods enable efficient isolation of cell surface proteins for further analysis and drug discovery.

Keywords:
BiotinCell surface proteinsElectrophoresisSDS-PAGEStreptavidin

More Related Videos

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Published on: July 3, 2017

10.1K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.9K

Related Experiment Videos

Last Updated: Feb 2, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
12:03

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Published on: July 23, 2017

9.9K
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Published on: July 3, 2017

10.1K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.9K

Area of Science:

  • Biochemistry
  • Proteomics
  • Cell Biology

Background:

  • Cell surface proteins are crucial for physiological functions and are key targets for novel therapeutics, representing two-thirds of the druggable proteome.
  • Characterizing the cell surface proteome is challenging due to difficulties in purifying these proteins from contaminants.
  • Biotin-streptavidin interaction-based methods have significantly advanced cell surface protein research.

Purpose of the Study:

  • To describe techniques for cell surface protein biotinylation and purification.
  • To highlight the utility of avidin affinity chromatography for isolating cell surface proteins.
  • To enable downstream analysis of purified cell surface proteins.

Main Methods:

  • Cell surface biotinylation using commercially available reagents.
  • Capture of biotinylated proteins via avidin affinity chromatography.
  • Release of purified proteins for subsequent electrophoretic analysis.

Main Results:

  • Efficient purification of cell surface proteins is achievable through biotinylation and avidin capture.
  • The described methods facilitate the isolation of integral and associated membrane proteins.
  • Downstream analysis by electrophoresis is enabled for the purified cell surface proteome.

Conclusions:

  • Cell surface biotinylation coupled with avidin affinity chromatography provides an effective strategy for purifying cell surface proteins.
  • This approach overcomes previous challenges in isolating these vital proteins.
  • The purified proteins are suitable for comprehensive downstream characterization, advancing drug discovery and physiological understanding.