Characterization of human tyrosinase ectodomain expressed in Escherichia coli
Ji-Na Kong1, Hee-Jin Lee, Dong-Hyun Jo
1Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University, Seoul, 156-756, Korea.
Protein and Peptide Letters
|April 17, 2010
Abstract:
The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.
