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[Abnormal expression of microRNAs in the hippocampus of Ts65Dn mice]
Yu-jun Zhang1, Xiang-jun He, Yu-jing Liu
1Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China.
Objective:
To identify the differentially expressed microRNAs in the hippocampus of Down Syndrome model mouse Ts65Dn.
Methods:
Low molecular weight RNA from hippocampus were tailed and reverse transcribed by extended RT-primer. MicroRNAs primers were arrayed on plates according to the Tm of each primer. PCR were carried out at different annealing temperatures using a gradient real-time PCR instrument. The relative expression level of each microRNAs was calculated using the most stable reference gene as normalizer which was selected by geNorm and Normfinder software.
Results:
miR-27a was identified as the most stable reference gene by geNorm and Normfinder software. Among the 52 microRNAs detected by real-time PCR array, miR-33 and miR-19a were significantly down-regulated, whereas miR-130 were significantly up-regulated in the hippocampus of Ts65Dn mice as compared with the euploid control mice. The expression of miR-802, which was the trisomy chromosome 16 derived microRNAs, was very low in hippocampus with no difference between the two groups.
Conclusion:
MicroRNAs are dysregulated in the hippocampus of Ts65Dn mice, which may contribute to the reduced neurogenesis of Down syndrome.
Insights
Down syndrome model mice (Ts65Dn) show altered microRNA expression in the hippocampus. Specific microRNAs (miR-33, miR-19a, miR-130) were found to be dysregulated, potentially impacting neurogenesis.
Area of Science:
- Molecular Biology
- Neuroscience
- Genetics
Context:
- Down syndrome is associated with cognitive deficits, partly due to hippocampal abnormalities.
- The Ts65Dn mouse is a widely used model for Down syndrome research.
- MicroRNAs play crucial roles in gene regulation and neuronal development.
Purpose:
- To investigate microRNA expression profiles in the hippocampus of Ts65Dn mice.
- To identify specific microRNAs that are differentially expressed in this Down syndrome model.
Summary:
- MicroRNA expression was analyzed in the hippocampus of Ts65Dn mice using real-time PCR array.
- miR-27a was validated as a stable reference gene.
- Significant downregulation of miR-33 and miR-19a, and upregulation of miR-130 were observed in Ts65Dn mice compared to controls.
- Expression of miR-802, a trisomic microRNA, was low and not significantly different between groups.
Impact:
- These findings reveal microRNA dysregulation in the Ts65Dn mouse hippocampus.
- Altered microRNA levels may contribute to the reduced neurogenesis observed in Down syndrome.
- This study provides insights into the molecular mechanisms underlying Down syndrome neuropathology.
