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Published on: January 1, 2018
[Suppression effect of ketamine on transient outward potassium currents in rathippocampal neurons]
Hong-yu Tan1, Li-na Sun, Xiao-liang Wang
1Key laboratory of Carcinogenesis and Translational Research, Ministry of Education, Department of Anesthesiology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
Objective:
To investigate the effect of ketamine on the transient outward potassium currents [IA] using whole-cell patch clamp technique.
Methods:
Pyramidal neurons were enzymatically isolated from Wistar rat hippocampus. The effect of ketamine on the [IA] was assessed using whole-cell patch clamp technique. Different concentrations of ketamine were added and potassium currents were measured.
Results:
[IA] was inhibited by ketamine in a concentration-dependent manner. 10 micromol/L ketamine had no effect on [IA]. The four concentrations of ketamine (30, 100, 300, 1000 micromol/L) reduced peak [IA] currents by (11+/-2)%, (22+/-3)%, (45+/-5)%, (53+/-5)% respectively, with a mean IC50 of (130+/-24) micromol/L and Hill coefficient of 1.19+/-0.56. The V1/2 of activation curve was shifted from (-8.70+/-0.13) mV to (-11.2+/-2.10) mV (n=8, P<0.05). The V1/2 of inactivation curve was shifted from (-75.53+/-7.98) mV to (-91.94+/-11.85) mV (n=8, P<0.05).
Conclusion:
Ketamine produces significant inhibition of potassium currents in the central neurons, which may least partly explain at the action of ketamine.
