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Published on: November 1, 2018
[Detection of Coxsackie B3 virus infection in newborn care unit using reverse transcriptase polymerase chain reaction
Wen E1, Shu-ping Liu, Jie Zheng
1Department of Pathology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Objective:
To explore the sensitivity of reverse transcriptase polymerase chain reaction (RT-PCR) method for detection of Coxsackie virus B3 (CoxB3) infection.
Methods:
RT-PCR method was used to detect CoxB3 viral RNA in peripheral blood samples of 31 sick children and their mothers (62 cases) from a single hospital.
Results:
Among the 62 samples taken from 31 sick children and their mothers, the serological test showed only one positive case in anti-Coxsackie virus IgM. The CoxB3 serological positive rate was 1.61%. The results of RT-PCR method showed that among the 62 samples there were 12 positive cases and the positive rate was 19.35%; both mothers and children were positive in four pairs of the samples (8 cases), suggesting vertical viral transmission; two cases of neonatal were positive with their mothers negative; two cases of mothers were positive with their newborns negative.
Conclusion:
RT-PCR is a rapid, sensitive and specific method for detection of CoxB3 infection and its positive rate is superior to serological method.
