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Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

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Related Experiment Video

Updated: Jun 13, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Cell-cycle markers and biosensors.

Laetitia Kurzawa1, May C Morris

  • 1Université de Montpellier, Montpellier, France.

Chembiochem : a European Journal of Chemical Biology
|April 17, 2010
PubMed
Summary
This summary is machine-generated.

Researchers have developed advanced fluorescent biosensors and genetically encoded markers for real-time cell-cycle analysis in living cells. These tools offer high-resolution insights into cell cycle regulation and potential diagnostic applications.

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Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
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Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Published on: May 9, 2019

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Last Updated: Jun 13, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
12:34

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Published on: May 9, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Classical cell cycle analysis relies on cell fixation, limiting real-time observation.
  • Green fluorescent protein (GFP) technology allows visualization of proteins in living cells.
  • Advancements in fluorescence and imaging enable detailed study of cellular processes.

Purpose of the Study:

  • To review traditional cell fixation methods for cell cycle status determination.
  • To describe the development and application of genetically encoded fluorescent markers.
  • To highlight the utility of fluorescent biosensors for studying cell cycle regulators in real-time.

Main Methods:

  • Review of classical cell fixation techniques.
  • Description of genetically encoded fluorescent protein fusions.
  • Exploration of fluorescent biosensor technology for probing cell cycle regulators.

Main Results:

  • Genetically encoded markers and fluorescent biosensors enable real-time, high-resolution monitoring of cell cycle status.
  • Biosensors allow in vivo investigation of cell cycle regulator dynamics.
  • These technologies facilitate comparative studies in healthy and pathological conditions.

Conclusions:

  • Fluorescent biosensors and markers revolutionize cell cycle research by enabling live-cell, high-resolution analysis.
  • These tools have potential applications in diagnostics and monitoring therapeutic interventions.
  • The study emphasizes the shift from fixed-cell to real-time analysis in cell cycle research.