[Methods for determining matrix metalloproteinase activity]

The paper describes methods for determining the activity of matrix metalloproteinases (MMP), primarily collagenases and gelatinases, by applying natural protein substrates. Reconstructed fluorescein-labeled type I collagen fibrils were used to determine collagenases as a substrate. The zymographic technique in polyacrylamide gel with copolymerized gelatin was employed for the identification and assay of gellatinases. These methods are rather sensitive, reproducible and may be used for screening. Collagenases specifically trigger the hydrolysis of fibrillar collagens; gelatinases are responsible for the hydrolysis of type IV collagen, the basis of basement membranes. MMP activity characterizes the development of a destructive or invasive process in the connective tissue matrix; this indicator is borne in mind when choosing therapeutic agents, has a prognostic value, determines targets for the development of pharmacological agents, and is required to understand the mechanism responsible for the destruction of matrix and for the development of invasion processes.