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Related Experiment Video

Updated: Jun 13, 2026

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

Elongator, a conserved multitasking complex?

Wim Versées¹, Steven De Groeve, Mieke Van Lijsebettens

¹Structural Biology Brussels, Vrije Universiteit Brussel, B-1050 Brussels, Belgium.

Molecular Microbiology

|April 20, 2010

Summary

The Elongator complex modifies transfer RNA (tRNA) wobble uridine, ensuring accurate codon decoding. This tRNA modification role is conserved across eukaryotes, including plants like Arabidopsis thaliana.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Post-transcriptional modifications of transfer RNA (tRNA) are crucial for translational regulation.
Specific modifications on tRNA wobble uridine, like methoxycarbonylmethyl (mcm) and carbamoylmethyl (ncm) groups, ensure accurate decoding of codons.

Purpose of the Study:

To investigate the conserved role of the Elongator complex in tRNA modification in the plant Arabidopsis thaliana.
To explore the functional complementation of Elongator subunits between yeast and plants.

Main Methods:

Investigating the Elongator complex's function in tRNA modification in Arabidopsis thaliana.
Assessing the structural and functional complementation of Arabidopsis Elongator subunits in yeast deletion mutants.

Main Results:

The tRNA-modifying role of the Elongator complex is conserved in Arabidopsis thaliana.
Arabidopsis Elongator subunits can functionally complement yeast deletion mutants, substituting for tRNA modification activity.

Conclusions:

The Elongator complex plays a conserved role in translational control via tRNA modification across eukaryotes.
Elongator is a multitasking complex involved in both nuclear transcriptional activation and cytoplasmic translational control.