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Updated: Jun 13, 2026

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
Interaction between non-anionic phospholipids and cytochrome c induced by reactive oxygen species
Nazha Sidahmed-Adrar1, Catherine Marchetti, Dominique Bonnefont-Rousselot
1Equipe de Physico-Chimie des Espèces Radicalaires, CNRS UMR 8601, Université Paris Descartes, 45 rue des Saints-Pères, 75006 Paris, France.
Abstract:
The oxidative interaction of cytochrome c (Cyt c) with liposomes of Palmitoyl Linoleyl Phosphatidyl Choline (PLPC) initiated by radio-induced free radicals was investigated. Results showed that the peroxidation of PLPC is decreased in the presence of Cyt c, meaning that this latter is the preferential target of hydroxyl radicals. In addition, when Cyt c was incubated with peroxidized PLPC, it was found to be able to decompose hydroperoxides of PLPC into hydroxides. The peroxidase activity of Cyt c proceeded via the opening of the tertiary structure of Cyt c, as suggested by the loss of the sixth coordination bond of the heme-iron. Even if it is known to preferentially interact with cardiolipin, this work shows that Cyt c is also able to interact with hydroperoxide species of non-anionic phospholipids.
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