Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flame Photometry: Overview

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...

Flame Photometry: Lab

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...

Atomic Spectroscopy: Effects of Temperature

Atomic Spectroscopy: Effects of Temperature

Atomization, converting samples into gas-phase atoms and ions, is essential for atomic spectroscopy. The flame temperature required for atomization affects the efficiency of the atomic spectroscopic methods by increasing the atomization efficiency and the relative population of the excited and ground states.
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature from...

Atomic Fluorescence Spectroscopy

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

Atomic Emission Spectroscopy: Interference

Atomic Emission Spectroscopy: Interference

In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High genetic risk scores of SLIT3, PLEKHA5 and PPP2R2C variants increased insulin resistance and interacted with coffee and caffeine consumption in middle-aged adults.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2018

Same author

People with the major alleles of ATP2B1 rs17249754 increases the risk of hypertension in high ratio of sodium and potassium, and low calcium intakes.

Journal of human hypertension·2017

Same author

An optically accessible pyrolysis microreactor.

The Review of scientific instruments·2016

Same author

Ebselen pretreatment attenuates ischemia/reperfusion injury and prevents hyperglycemia by improving hepatic insulin signaling and β-cell survival in gerbils.

Free radical research·2014

Same author

Gestational diabetes is associated with high energy and saturated fat intakes and with low plasma visfatin and adiponectin levels independent of prepregnancy BMI.

European journal of clinical nutrition·2013

Same author

Anti-obesity effect of kimchi fermented with Weissella koreensis OK1-6 as starter in high-fat diet-induced obese C57BL/6J mice.

Journal of applied microbiology·2012

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Experimental Methodology for Estimation of Local Heat Fluxes and Burning Rates in Steady Laminar Boundary Layer Diffusion Flames

Experimental Methodology for Estimation of Local Heat Fluxes and Burning Rates in Steady Laminar Boundary Layer Diffusion Flames

Published on: June 1, 2016

Two-line atomic fluorescence temperature measurement in flames: an experimental study.

R G Joklik¹, J W Daily

¹University of California, Department of Mechanical Engineering, Berkeley, California 94720, USA.

|April 20, 2010

Summary

This study demonstrates the feasibility of a high-speed two-line atomic fluorescence (TLF) system for combustion research. The developed TLF system shows potential for accurate temperature measurements in turbulent flows.

More Related Videos

Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes

Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes

Published on: May 26, 2014

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Published on: February 19, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Experimental Methodology for Estimation of Local Heat Fluxes and Burning Rates in Steady Laminar Boundary Layer Diffusion Flames

Experimental Methodology for Estimation of Local Heat Fluxes and Burning Rates in Steady Laminar Boundary Layer Diffusion Flames

Published on: June 1, 2016

Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes

Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes

Published on: May 26, 2014

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Published on: February 19, 2018

Area of Science:

Combustion Science
Atomic Physics
Laser Spectroscopy

Background:

Traditional temperature measurement techniques in combustion are limited in speed.
Turbulent flow requires high-speed diagnostic tools for accurate analysis.
Two-line atomic fluorescence (TLF) offers potential for high-repetition-rate measurements.

Purpose of the Study:

To investigate the feasibility of a high-speed TLF system for combustion diagnostics.
To develop a TLF system capable of 10 kHz data acquisition.
To assess and improve the accuracy and precision of TLF temperature measurements.

Main Methods:

Utilized indium seeded into a flat-flame methane burner.
Employed dye-laser excitation sources for high-speed measurements.
Incorporated a computer data acquisition system for rapid data collection.
Focused on the 410- and 451-nm transitions of indium.

Main Results:

Preliminary one-shot measurements achieved approximately 13% precision and 350 K accuracy.
Identified major sources of experimental error in the TLF system.
Demonstrated the capability for high-speed data acquisition.

Conclusions:

The developed TLF system is feasible for high-speed combustion diagnostics.
Recommendations for error reduction are provided.
Expected precision and accuracy of better than 2% at high data rates are anticipated.