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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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Distributed simple sequence repeat markers for efficient mapping from maize public mutagenesis populations.

Federico Martin1, Sarah Dailey, A Mark Settles

  • 1Horticultural Sciences Department, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611-0690, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|April 20, 2010
PubMed
Summary

Researchers developed a set of 85 molecular markers for efficient genetic mapping in maize (Zea mays). This tool aids in rapidly identifying genes responsible for traits in public mutant populations, accelerating plant genetic research.

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Area of Science:

  • Plant Genetics
  • Molecular Biology
  • Genomics

Background:

  • The availability of maize (Zea mays) genome sequences facilitates map-based cloning of genes controlling various traits.
  • Publicly funded mutagenesis projects are generating valuable mutant resources, primarily in the W22 and B73 maize inbred lines.
  • Developing efficient mapping platforms for these genetic backgrounds is crucial for analyzing these resources.

Purpose of the Study:

  • To identify and validate a set of simple sequence repeat (SSR) markers for robust genetic mapping in maize.
  • To establish a nearly complete genetic map coverage across three key maize inbred lines (B73, Mo17, W22).
  • To demonstrate the utility of these markers for rapid mutant characterization in public maize populations.

Main Methods:

  • Screened 505 SSR markers for polymorphisms among B73, Mo17, and W22 maize inbreds.
  • Identified 85 SSR markers exhibiting co-dominant polymorphisms suitable for mapping.
  • Assessed marker distribution and density to ensure comprehensive genetic map coverage.

Main Results:

  • 47.1% of screened SSR markers displayed polymorphisms between at least one pair of inbred lines.
  • The selected 85 markers provide nearly complete genetic map coverage for all three inbred line pairs.
  • The marker set achieved a mean distance of 27-29 centimorgans (cM) between markers for each inbred pair.
  • Successfully demonstrated the marker set's efficacy in mapping a seed mutant from the UniformMu resource.

Conclusions:

  • The developed set of distributed molecular markers significantly enhances the efficiency of mapping phenotypic variations in public maize mutagenesis populations.
  • These markers provide a valuable tool for accelerating molecular genetic analysis and gene discovery in maize.
  • Facilitates rapid characterization of mutants, aiding in the understanding of maize genetics and trait development.