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Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using

Melissa H Pespeni1, Thomas A Oliver, Mollie K Manier

  • 1Department of Biology, Stanford University, Hopkins Marine Station, Oceanview Blvd Pacific Grove, CA 93950, USA. mpespeni@stanford.edu

Genome Biology
|April 21, 2010
PubMed

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...