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Updated: Jun 13, 2026
Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility
Published on: April 5, 2024
Serum anti-PDIK1L autoantibody as a novel marker for endometriosis
Motowo Nabeta1, Yasuhito Abe, Ryuma Haraguchi
1Department of Molecular Pathology, Ehime University Graduate School of Medicine, Ehime, Japan.
Objective:
To establish a novel serum marker for endometriosis, serum autoantibodies (autoAbs) were investigated using a proteomic approach.
Design:
Retrospective study.
Setting:
Departments of Molecular Pathology and Obstetrics and Gynecology in Ehime University and University Hospital.
Patient(S):
Sixty-nine patients with endometriosis, 38 disease control patients without endometriosis, and 44 healthy volunteers.
Intervention(S):
Autoantibodies in sera of endometriotic patients and healthy controls were investigated using a human fibroblast cell line, two-dimensional gel electrophoresis, and Western blotting. Proteins in reacted spots were identified using MALDI time of flight mass spectrometry with MASCOT analysis. ELISAs were established using recombinant proteins, and autoAb-titers were estimated in sera of endometriotic patients and controls.
Main Outcome Measure(S):
Identification of serum autoAb useful for diagnosis of endometriosis.
Result(S):
Several autoAbs were identified. ELISAs were established and serum autoAb titers were estimated. Among those identified, anti-PDIK1L-autoAb levels were significantly elevated in endometriotic patients. Sensitivity (59.4%) and accuracy (72.8%) of serum anti-PDIK1L-autoAb assay were better than those of serum CA125 levels (36.2% and 62.9%, respectively) in diagnosis of endometriosis. Additionally, anti-PDIK1L-autoAb could detect endometriotic patients in early stages.
Conclusion(S):
Serum anti-PDIK1L-autoAb can be a new serum marker for the diagnosis of endometriosis. This study validates further clinical evaluation of this novel marker.
