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Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Adaptability of Cytoskeletal Filaments01:12

Adaptability of Cytoskeletal Filaments

The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jun 13, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Cytoskeletal dynamics and spermatogenesis.

Pearl P Y Lie1, Dolores D Mruk, Will M Lee

  • 1The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|April 21, 2010
PubMed
Summary
This summary is machine-generated.

The cytoskeleton plays a crucial role in male germ cell development and movement during spermatogenesis. Understanding its dynamics is key for developing male contraception and treating reproductive dysfunction.

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Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
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Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
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Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
10:30

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Published on: January 20, 2014

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Spermatogenesis involves complex cellular events like mitosis, meiosis, and spermiogenesis.
  • The cytoskeleton, composed of actin, microtubules, and intermediate filaments, is implicated in these processes.
  • Limited research has explored the cytoskeleton's specific roles in spermatogenesis.

Purpose of the Study:

  • To review recent advances in cytoskeletal dynamics during spermatogenesis.
  • To identify research gaps and future directions in this field.
  • To explore potential applications in male contraception and reproductive health.

Main Methods:

  • Literature review of recent studies on cytoskeletal dynamics in the testis.
  • Synthesis of current knowledge on cytoskeleton involvement in germ cell events.
  • Identification of emerging research areas and their significance.

Main Results:

  • The cytoskeleton is essential for cell shape changes, movement, and progression through spermatogenesis.
  • Specific cytoskeletal components play distinct roles in mitosis, meiosis, and spermiogenesis.
  • Environmental toxicants can disrupt cytoskeletal function, impacting male fertility.

Conclusions:

  • Further research on cytoskeletal dynamics is crucial for understanding male reproductive health.
  • Targeting cytoskeletal functions may lead to novel male contraceptive strategies.
  • Understanding these mechanisms can help in developing therapies for environmentally induced male reproductive dysfunction.