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A new label-free continuous fluorometric assay for trypsin and inhibitor screening with tetraphenylethene compounds
Wangxin Xue1, Guanxin Zhang, Deqing Zhang
1Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Abstract:
A new label-free continuous assay with the ensemble of compound 1 and Arg(6) for trypsin and inhibitor screening was successfully developed by taking advantage of the aggregation-induced emission feature of tetraphenylethene compounds. Both CLSM and DLS studies confirm the formation of the aggregation complex of compound 1 and Arg(6) and disassembly after further addition of trypsin.
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