Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic birds and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stable, micron-scale lipocondensates achieving prolonged circulation without PEGlyated lipids.

Biomaterials science·2026
Same author

Lipid-Protein Membrane Structure-Function Characterization using Droplet Interface Bilayers.

Journal of visualized experiments : JoVE·2026
Same author

Melatonin-Induced Modulation of Cholesterol-Enriched Model Neuronal Membranes.

ACS chemical neuroscience·2026
Same author

Ion transport through reconfigurable nanoparticle-surfactant stabilized droplet interface bilayers.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Spontaneous Graphene/Lipid Bicellar Co-assembly (NANO<sup>2</sup>-Graphene): Experiments and Computer Simulations.

ACS applied materials & interfaces·2025
Same author

Electromechanically induced membrane restructuring enables learning and memory.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Fiber-reinforced hydrogels: From multiscale structural design to advanced engineering applications.

Biointerphases·2026
Same journal

Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.

Biointerphases·2026
Same journal

Biointerfaces in India.

Biointerphases·2026
Same journal

Biomimetic illumination enhancement inspired by guanine platelets in the photophore surface of the deep-sea bristlemouth Sigmops gracilis.

Biointerphases·2026
Same journal

Binding and orientation of ice nucleating proteins on hydrophilic and hydrophobic surfaces probed by photoelectron spectroscopies.

Biointerphases·2026
Same journal

Shell damage and mandible mechanics in the ant Messor wasmanni.

Biointerphases·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Structure from substrate supported lipid bilayers (Review).

John Katsaras1, Norbert Kucerka, Mu-Ping Nieh

  • 1Canadian Neutron Beam Centre, Steacie Institute for Molecular Sciences, National Research Council, Chalk River Laboratories, Chalk River, Ontario, Canada K0J 1J0. john.katsaras@nrc.gc.ca

Biointerphases
|April 23, 2010
PubMed
Summary
This summary is machine-generated.

Substrate-supported membranes enable precise structural analysis of model membranes. This advancement overcomes limitations of traditional membrane dispersions, revealing new insights into membrane structure.

More Related Videos

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Related Experiment Videos

Last Updated: Jun 13, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Area of Science:

  • Biophysics
  • Materials Science
  • Structural Biology

Background:

  • Membrane structure analysis is crucial for understanding biological functions.
  • Traditional methods using membrane dispersions (powder samples) yield limited structural information.
  • Substrate-supported membranes offer a novel approach for detailed structural investigation.

Purpose of the Study:

  • To review major breakthroughs in model membrane research.
  • To highlight the impact of substrate-supported samples on structural analysis.
  • To showcase advancements in understanding membrane structure.

Main Methods:

  • Utilizing physical techniques for structural determination.
  • Employing highly aligned, substrate-supported membrane models.
  • Comparing results with traditional membrane dispersion methods.

Main Results:

  • Unambiguous structural information is now accessible.
  • Significant progress has been made in model membrane research.
  • Substrate-supported samples provide superior data quality.

Conclusions:

  • Substrate-supported membranes are transformative for membrane research.
  • This technique allows for unprecedented insights into membrane structure.
  • Future studies will benefit from these advanced sample preparations.