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Microcontact Printing of Proteins for Cell Biology
Published on: December 5, 2008
Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
Jens A Wigenius1, Sophia Fransson, Fredrik von Post
1Biomolecular and Organic Electronics, Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden.
Biointerphases
|April 23, 2010
Summary
Soft lithography stamps enable simple protein patterning for antibody biochips. Microcontact printing transfers poly(dimethylsiloxane) (PDMS), potentially causing unspecific protein adsorption to hydrophobic surfaces.
Area of Science:
- Biotechnology
- Materials Science
- Surface Chemistry
Background:
- Protein patterning is essential for developing advanced protein biochips.
- Established methods include layer printing and surface adsorption onto functionalized surfaces.
Purpose of the Study:
- To demonstrate simple methods for preparing functional antibody chips using soft lithography.
- To investigate protein patterning via both printing and adsorption routes.
Main Methods:
- Utilized soft lithography stamps for protein patterning.
- Employed microcontact printing and surface adsorption techniques.
- Analyzed the transfer of stamp material, poly(dimethylsiloxane) (PDMS), during the process.
Main Results:
- Successfully prepared functional antibody chips using soft lithography.
- Observed consistent PDMS transfer during microcontact printing of proteins.
- Identified the potential for unspecific protein adsorption onto transferred PDMS hydrophobic domains.
Conclusions:
- Soft lithography offers a straightforward approach for antibody chip fabrication.
- The inherent PDMS transfer in microcontact printing necessitates consideration of its impact on biochip performance.
- Understanding PDMS transfer is crucial for optimizing protein immobilization and preventing non-specific binding in biochip applications.
